In Vitro Culture and Neural Differentiation of Human Urine-derived Stem Cells
Received:November 01, 2018  Revised:December 24, 2018
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Key words:Human urine-drived stem cells, neuron cell, neuroglia cell, differentiation
Author NameAffiliationE-mail
JIANG Zhi-Xin 第六人民医院内分泌代谢科室 jiangzhixin1992@163.com 
WANG Cong-Rong 第六人民医院内分泌代谢科室 crwang@sjtu.edu.cn 
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Abstract:
      Objective Human urinary stem cells (hUSCs) with mesenchymal stem cells (MSCs) characteristics were isolated from urine of healthy individuals, and its neurogenic differentiation capabilities in different neurogenic induction mediums were investigated. Methods HUSCs from normal individuals were extracted, cultured, and identified. Four different neurogenic differentiation methods were used. After induction for 14 days, cell morphology was observed under the microscope, and differentiation capability was identified by RT-PCR. Results HUSCs extracted from healthy adults successfully expressed MSCs surface markers (CD27+, CD44+, CD73+, CD90+, CD31-, CD34-, CD45-, HLA-DR-). After 14 days induction in B, C, D neural differentiation medium, hUSCs showed a retraction of the cell body and high refracting power, as well as processing neuron-like protuberance. However, RT-PCR showed relative markers were not detectable in medium A after 14 days induction. The expression of Nestin, a specific marker of neural stem cells (NSCs), increased significantly in medium B、C、D (Ps < 0.01), especially in medium C(95.63±3.79); The expression of S100, a specific marker of percursor cells of oligodendrocytes and astrocytes also increased in medium B、C、D (Ps < 0.01), among which the effect of medium B was better(6.15±0.18); The expression of neuron cell-specific markers β3-tublin and NF-200 were not increased in all medium. Conclusion HUSCs possessed MSCs characteristics. The B, C, D neural differentiation medium contributed to the differentiation of hUSCs into neuron cells. The results suggest that hUSC can differentiate into NSC and glial subpopulations under optimized induction conditions.
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